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Neurofilament M (NF-M) Antibody

Mouse monoclonal antibody

  • WB - Neurofilament M (NF-M) Antibody AN1148
    Western blot of rat cortex lysate showing specific immunolabeling of the ~ 145k NF-M protein.
  • IF - Neurofilament M (NF-M) Antibody AN1148
    Immunostaining of cultured rat neurons showing labeling of NF-M (green) in mature neurons.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12839
Reactivity Rat
Predicted Chicken, Human, Mouse
Host Mouse
Clonality monoclonal
Isotype IgG1
Clone Names 3H11
Calculated MW 145 KDa
Additional Information
Gene ID 24588
Gene Name NEFM
Other Names Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, Nefm, Nef3, Nfm
Target/Specificity Preparation containing the extreme C-terminus expressed in and purified from E. Coli.
Dilution WB~~ 1:1000
IF~~ 1:100
Format Unpurified, concentrated culture supernatant.
Antibody Specificity Specific for the ~145k neurofilament M protein.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNeurofilament M (NF-M) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
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Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2).


Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.
Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60.

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$ 285.00
Cat# AN1148
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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