|Application ||IF, WB|
|Reactivity||Bovine, Human, Mouse, Rat|
|Calculated MW||50 KDa|
|Other Names||Vimentin, VIM|
|Target/Specificity||Recombinant human vimentin purified from E. coli.|
|Dilution||WB~~ 1:1000 |
|Format||Total IgY fraction.|
|Antibody Specificity||Specific for the ~50kDa Vimentin protein.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Vimentin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).
Nieminen M, Henttinen T, Merinen M, Marttila-Ichihara F, Eriksson JE, Jalkanen S (2006)
Vimentin function in lymphocyte adhesion and transcellular migration. Nat Cell Biol
Zhao Y, Yan Q, Long X, Chen X, Wang Y (2008) Vimentin affects the mobility and invasiveness of prostate cancer cells. Cell Biochem Funct. May 8 [Epub ahead of print]
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