|Application ||WB, IF|
|Calculated MW||80 KDa|
|Other Names||Myristoylated alanine-rich C-kinase substrate, MARCKS, Protein kinase C substrate 80 kDa protein, Marcks, Macs|
|Target/Specificity||Recombinant protein expressed in and purified from E. coli.|
|Format||Unpurified neat serum.|
|Antibody Specificity||Specific for the ~80k MARCKS protein|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MARCKS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al.,1990) and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro (Hartwig et al. 1992). Deletion of the MARCKS gene in mice results in embryonic brain defects and death (Stumpo et al. 1995). MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).
Ouimet CC, Wang JKT, Walaas SI, Albert KA, Greengard P (1990) Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain. J Neurosci 10:1683-1698.
Hartwig JH, Thelen M, Rosen A, Janmey PA, Nairn AC and Aderem A. (1992) MARCKS is an a ctin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 356: 618-622
Stumpo DJ, Bock CB, Tuttle JS and Blackshear PJ. (1995) MARCKS deficiency in mice leads to abnormal brain development and perinatal death. Proc. Nat. Acad. Sci. 92; 944-948.
Verghese GM, Johnson JD, Vasulka C, Haupt DM, Stumpo DJ, Blackshear PJ (1994) Protein
kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated
alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. J Biol Chem
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