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Microtubule Associated Protein 2 (MAP2) Antibody

Chicken polyclonal antibody

  • WB - Microtubule Associated Protein 2 (MAP2) Antibody AN1161
    Western blot of rat cortex lysate showing specific immunolabeling of the ~280k MAP2protein.
  • IF - Microtubule Associated Protein 2 (MAP2) Antibody AN1161
    Mixed neuron/glial cultures. The perikarya and dendrites of neurons are stronglyand specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11137
Reactivity Bovine, Human, Mouse, Rat
Host Chicken
Clonality polyclonal
Calculated MW 280 KDa
Additional Information
Gene ID 4133
Gene Name MAP2
Other Names Microtubule-associated protein 2, MAP-2, MAP2
Target/Specificity Bovine MAP2 isolated from brain by the GTP microtubule cycling method.
Dilution WB~~ 1:20000
IF~~ 1:2500
Format Total IgY fraction
Antibody Specificity Specific for the ~ 280k MAP2 protein.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMicrotubule Associated Protein 2 (MAP2) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
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Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).


Kindler S, Gardner CC (1994) Four repeat MAP2 isoforms in human and rat brain. Brain Res
Mol Brain Res. 26(1-2):218-224.
Fontaine-Lenore V, Chambraud B, Fellous A, David S, Duchossoy Y, Baulieu EE, Robel P
(2006) Microtubule-associated protein 2 (MAP2) is a neurosteroid. Proc Natl Acad Sci USA

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$ 285.00
Cat# AN1161
(40 western blots)
Availability: 7-10 days
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