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Phospho-Ser260 Tryptophan Hydroxylase Antibody

Affinity purified rabbit polyclonal antibody

     
  • WB - Phospho-Ser260 Tryptophan Hydroxylase Antibody AN1174
    Western blot of rat brainstem lysate showing specific immunolabeling of the ~55k TPH protein phosphorylated at Ser260. The labeling is specifically blocked by the phosphopeptide (Phos-pep) used as antigen. The corresponding non-phosphopeptide (Nonphos-pep) did not block the immunolabeling.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P09810
Reactivity Human, Rat
Predicted Bovine, Chicken, Mouse, Zebrafish
Host Rabbit
Clonality polyclonal
Calculated MW 55 KDa
Additional Information
Gene ID 24848
Gene Name TPH1
Other Names Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1, Tph1, Tph
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Antibody Specificity Specific for the ~55k tryptophan hydroxylase protein phosphorylated at Ser260.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser260 Tryptophan Hydroxylase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
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Background

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

References

Jiang GC, Yohrling GJ, Schmitt JD, Vrana KE (2000) Identification of substrate orienting and phosphorylation sites within tryptophan hydroxylase using homology-based molecular modeling. J Mol Biol 302:1005-1017.
Johansen PA, Jennings I, Cotton RG, Kuhn DM (1996) Phosphorylation and activation of tryptophan hydroxylase by exogenous protein kinase A. J Neurochem 66:817-823.
Martinez A, Knappskog PM, Haavik J (2001) Structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis. Curr Med Chem 8:1077-1091.

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$ 335.00
Cat# AN1174
Size:
Quantity:
(40 western blots)
Availability: 7-10 days
Bulk Size
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