|Application ||WB, IF|
|Reactivity||Bovine, Human, Mouse, Rat|
|Calculated MW||18/22 KDa|
|Other Names||Myelin basic protein, MBP, Mbp|
|Target/Specificity||Three peptide sequences conserved in higher vertebrate MBP proteins.|
|Dilution||WB~~ 1:5000 |
|Format||Total IgY fraction.|
|Antibody Specificity||Specific for the ~18k & 22k myelin basic protein in Western blots of ratbrain homogenate|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Myelin Basic Protein (MBP) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (Eylar et al., 1971). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (Marty et al., 2002). In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22kDa and another at about 18kDa.
Eylar EH, Brostoff S, Hashim G, Caccam J, Burnett P. (1971) Basic A1 protein of the myelin membrane: the complete amino acid sequence. J. Biol. Chem. 246:5770-5784.
Marty MC, Alliot F, Rutin J, Fritz R, Trisler D. and Pessac B. (2002) The myelin basic protein gene is expressed in differentiated blood cell lineages and in hematopoietic progenitors. Proc. Nat. Acad. Sci. 99:8856-8861.
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