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Vesicular Monoamine Transporter 2, C-Terminus Antibody

Affinity purified sheep polyclonal antibody

     
  • WB - Vesicular Monoamine Transporter 2, C-Terminus Antibody AN1209
    Western blot of rat caudate lysate showing specific immunolabelingof the ~ 57k VMAT2 protein.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q05940
Reactivity Rat
Host Sheep
Clonality polyclonal
Calculated MW 57 KDa
Additional Information
Gene ID 6571
Gene Name VMAT2
Other Names Synaptic vesicular amine transporter, Monoamine transporter, Solute carrier family 18 member 2, Vesicular amine transporter 2, VAT2, SLC18A2, SVMT, VMAT2
Target/Specificity Synthetic peptide corresponding to amino acid residues from the intracellular C-terminal region conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from sheep serum by affinity purification using a column to which the peptide immunogen was coupled.
Antibody Specificity Specific for the ~57k VMAT2 protein in Western blots of rat caudate lysate.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVesicular Monoamine Transporter 2, C-Terminus Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
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citation

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Background

Vesicular neurotransmitter transporters sequester the transmitters into synaptic vesicles (Erickson et al., 1996). The vesicular monoamine transporter 2 (VMAT2) is responsible for catecholamine and serotonin storage in central synapses. Antibodies specific for VMAT have been used to monitor expression of the transporter during development and in aging and can be effectively used as a marker for monoamine terminals (Haycock et al., 2003; Witkovsky et al., 2005).

References

Erickson JD, Schafer MKH, Bonner TI, Eiden LE, Weihe E (1996) Distinct pharmacological properties and distribution in neurons and endocrine cells of two isoforms of the human vesicular monoamine transporter. Proc Natl Acad Sci USA 93:5166-5171.
Giros B, El Mestikawy S, Godinot N, Zheng K, Han H, Yang-Feng T, Caron MG (1991) Cloning, pharmacological characterization, and chromosome assignment of the human dopamine transporter. J Pharmacol Exptl Ther 56:2139-2142.
Haycock JW, Becker L, Ang, L, Furukawa Y, Hornykiewicz O, Kish SJ (2003) Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J Neurochem 87:574-585.
Witkovsky P, Arango-Gonzalez B, Haycock JW, Kohler K (2004) Rat retinal dopaminergic neurons: Differential maturation of somatodendritic and axonal compartments. J Comp Neurol 481:352-362.

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$ 365.00
Cat# AN1209
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