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Phospho-Ser940 Potassium Chloride Cotransporter (KCC2) Antibody

Affinity purified rabbit polyclonal antibody

     
  • FC - Phospho-Ser940 Potassium Chloride Cotransporter (KCC2) Antibody AN1210
    Flow cytometric analysis of PC-12 cells using Park7 (DJ-1) Antibody(green, Cat#AN1210) compared to an isotype control of rabbit IgG(blue). AN1210 was diluted at 1:500 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
    detail
  • WB - Phospho-Ser940 Potassium Chloride Cotransporter (KCC2) Antibody AN1210
    Western blot of rat hippocampal homogenate showing specific labeling ofthe ~ 135k KCC2 protein (Control). Immunolabeling is blocked bypreadsorption with the phospho-peptide used as antigen (Peptide) butnot by the corresponding dephospho-peptide (not shown).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB
Primary Accession Q63633
Reactivity Rat
Predicted Bovine, Human, Mouse, Monkey
Host Rabbit
Clonality polyclonal
Calculated MW 135 KDa
Additional Information
Gene ID 171373
Gene Name KCC2
Other Names Solute carrier family 12 member 5, Electroneutral potassium-chloride cotransporter 2, Furosemide-sensitive K-Cl cotransporter, K-Cl cotransporter 2, rKCC2, Neuronal K-Cl cotransporter, Slc12a5, Kcc2
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 conjugated to KLH.
Dilution FC~~1:500
WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns.
Antibody Specificity Specific for the ~135k KCC2 protein phosphorylated at Ser940. Immunolabeling of the KCC2 protein band is blocked by the phospho-peptide used as antigen but not by the corresponding dephosphopeptide.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser940 Potassium Chloride Cotransporter (KCC2) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Background

KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser940 increased the cell surface stability of KCC2 in this system by decreasing it’s rate of internalization from the plasma membrane (Lee et al., 2007).

References

Lee HH, Walker JA, Williams JR, Goodier RJ, Payne JA, Moss SJ (2007) Direct protein kinase C-dependent phosphorylation regulates the cell surface stability and activity of the potassium chloride cotransporter KCC2. J Biol Chem. 2007 Oct 12;282(41):29777-84
Lee HH, Deeb TZ, Walker JA, Davies PA, Moss SJ (2011) NMDA receptor activity downregulates KCC2 resulting in depolarizing GABAA receptor-mediated currents. Nat Neurosci. Jun;14(6):736-43

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$ 365.00
Cat# AN1210
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