Phospho-Ser940 Potassium Chloride Cotransporter (KCC2) Antibody
Affinity purified rabbit polyclonal antibody
|Application ||FC, WB|
|Predicted||Bovine, Human, Mouse, Monkey|
|Calculated MW||135 KDa|
|Other Names||Solute carrier family 12 member 5, Electroneutral potassium-chloride cotransporter 2, Furosemide-sensitive K-Cl cotransporter, K-Cl cotransporter 2, rKCC2, Neuronal K-Cl cotransporter, Slc12a5, Kcc2|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns.|
|Antibody Specificity||Specific for the ~135k KCC2 protein phosphorylated at Ser940. Immunolabeling of the KCC2 protein band is blocked by the phospho-peptide used as antigen but not by the corresponding dephosphopeptide.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Ser940 Potassium Chloride Cotransporter (KCC2) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser940 increased the cell surface stability of KCC2 in this system by decreasing it’s rate of internalization from the plasma membrane (Lee et al., 2007).
Lee HH, Walker JA, Williams JR, Goodier RJ, Payne JA, Moss SJ (2007) Direct protein kinase C-dependent phosphorylation regulates the cell surface stability and activity of the potassium chloride cotransporter KCC2. J Biol Chem. 2007 Oct 12;282(41):29777-84
Lee HH, Deeb TZ, Walker JA, Davies PA, Moss SJ (2011) NMDA receptor activity downregulates KCC2 resulting in depolarizing GABAA receptor-mediated currents. Nat Neurosci. Jun;14(6):736-43
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