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Nestin Antibody

Mouse monoclonal antibody

  • WB - Nestin Antibody AN1216
    Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 k nestin doublet.
  • IF - Nestin Antibody AN1216
    Immunochemical staining of cultured neonatal rat neurons and glia showing nestin labeling in red and vimentin in green. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P48681
Reactivity Human, Mouse, Rat
Host Mouse
Clonality monoclonal
Isotype IgG1
Clone Names 4D11
Calculated MW 240 KDa
Additional Information
Gene ID 10763
Gene Name NES
Other Names Nestin, NES
Target/Specificity Recombinant human Nestin expressed in and purifeid from E. coli.
Dilution WB~~ 1:2000
IF~~ 1:500
Format Protein G purified antibody in 100 ul PBS + 10 mM NaN3
Antibody Specificity Specific for the ~220-240 kDa nestin doublet in Western blot in neonatal rat brain homogenate.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNestin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells (Lendahl et al., 1990). Nestin was originally identified as a result of the production of a series of monoclonal antibodies directed against epitopes expressed on formalin fixed embryo day 15 rat spinal cord (Hockfield et al., 1985)). One of these antibodies, called Rat 401, stained fibrous profiles in the developing nervous system, but not in the mature nervous system. By screening bacteriophage expression libraries with Rat 401, Lendahl et al. were able to isolate a cDNA encoding the protein to which Rat 401 antibody bound. The protein proved to be an unusual member of the intermediate filament family, containing an alpha-helical region homologous to that found in keratin and neurofilament subunits.


Lendahl U, Zimmerman LB and McKay RD. CNS stem cells express a new class of intermediate filament protein. Cell 60:585-95 (1990).
Hockfield S and McKay RD. Identification of major cell classes in the developing mammalian nervous system. J. Neurosci. 5:3310-3328 (1985).

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$ 285.00
Cat# AN1216
(40 western blots)
Availability: 7-10 days
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