Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
Phospho-Ser23/24 Troponin I (cardiac) Antibody
Affinity purified rabbit polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P48787 |
| Reactivity | Mouse, Rat |
| Predicted | Human, Monkey |
| Host | Rabbit |
| Clonality | polyclonal |
| Calculated MW | 25 KDa |
| Gene ID | 21954 |
|---|---|
| Gene Name | TNNI3 |
| Other Names | Troponin I, cardiac muscle, Cardiac troponin I, Tnni3 |
| Target/Specificity | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser23/24 conjugated to KLH. |
| Dilution | WB~~ 1:1000 |
| Format | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns. |
| Antibody Specificity | Specific for the ~25k cardiac troponin I protein phosphorylated at Ser23/24. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-Ser23/24 Troponin I (cardiac) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place, and when cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 it causes regulation of Ca2+- stimulated ATPase (Noland et al, 1995). Evidence suggests that phosphorylation of both serines 23 and 24 is required for the reduction in Ca2+-sensitivity and beneficial for relaxation of the heart (Kooij et al, 2010). Ser23/24 phosphorylation is important for enhanced relaxation in response to prolonged activation of protein kinase C by endothelin in intact myocytes, while Thr144 plays an important role in the acute acceleration of relaxation (Westfall et al, 2005).
References
Thomas A. Noland, Jr., Xiaodu Guo, Robert L. Raynor, Nathan M. Jideama, Vera Averyhart-Fullard, R. John Solaro, and J.F. Kuo (1995) Cardiac Troponin I Mutants. J of Biol Chem 270 (43): 25445-25454.
Viola Kooij, Martina Saes, Kornelia Jaquet, Ruud Zaremba, D. Brian Foster, Anne M. Murphy, Cris dos Remedios, Joland van der Velden, Ger J.M. Stienen (2010) Effect of Troponin I ser23/24 phosphorylation on Ca2+-sensitivity in human myocardium depends on the phosphorylation background. J of Mol Cell Card 48: 954-963.
Margaret V. Westfall, Adonia M. Lee, and Dustin A. Robinson (2005) Differential Contribution of Troponin I Phosphorylation Sites to
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
