|Predicted||Bovine, Chicken, Mouse, Monkey, Xenopus, Zebrafish|
|Calculated MW||60 KDa|
|Other Names||Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.|
|Antibody Specificity||Specific for the ~60k beclin1 protein phosphorylated at Ser295. Immunolabeling of the beclin-1 band is eliminated in the beclin-1 alanine substitution mutant. dditional bands may be observed in non-immunoprecipitated lysates.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Ser295 Beclin-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Akt-mediated phosphorylation of beclin-1 at Ser295 has been shown to decrease autophagy and promote oncogenesis (Wang et al., 2012).
Liang, XH, Jackson S, Seaman M, Brown K, Kempkes B, Hibshoosh H, Levine B.
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