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Phospho-Ser295 Beclin-1 Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Ser295 Beclin-1 Antibody AN1226
    Western blot of flag beclin-1 WT immunoprecipitates expressed in 293T cells shospecific immunolabeling of beclin-1 when phosphorylated at S295 (WT). This immunolabeling is eliminated in the beclin-1 alanine substitution mutant(S295A).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14457
Reactivity Human
Predicted Bovine, Chicken, Mouse, Monkey, Xenopus, Zebrafish
Host Rabbit
Clonality polyclonal
Calculated MW 60 KDa
Additional Information
Gene ID 8678
Gene Name BECN1
Other Names Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 conjugated to KLH.
Dilution WB~~ 1:250
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Antibody Specificity Specific for the ~60k beclin1 protein phosphorylated at Ser295. Immunolabeling of the beclin-1 band is eliminated in the beclin-1 alanine substitution mutant. dditional bands may be observed in non-immunoprecipitated lysates.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser295 Beclin-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Akt-mediated phosphorylation of beclin-1 at Ser295 has been shown to decrease autophagy and promote oncogenesis (Wang et al., 2012).


Liang, XH, Jackson S, Seaman M, Brown K, Kempkes B, Hibshoosh H, Levine B.

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$ 335.00
Cat# AN1226
(40 western blots)
Availability: 7-10 days
Bulk Size
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