|Application ||WB, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||78 KDa|
|Other Names||Synapsin-1, Synapsin I, SYN1|
|Target/Specificity||Native protein purified from bovine brain.|
|Format||Prepared from rabbit serum by affinity purification using a column to which the native protein was coupled.|
|Antibody Specificity||Specific for the ~78k synapsin I doublet in Western blots of rat brainextracts. Immunolabeling blocked by preadsorption of antibody with the protein used togenerate the antibody.|
|Precautions||Synapsin I Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).
Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Fe
rreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA,
Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372-
Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Gr
eengard P, Czernik AJ (2001) Opposing changes in
phosphorylation of specific sites in synapsin I during Ca
-dependent glutamate release in isolated nerve
terminals. J Neurosci 21:7944-7953.
Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M,
Czernik AJ, Pieribone VA, Poo MM, Greengard P (2002) A
protein kinase A-dependent molecular switch in synapsin
s regulates neurite outgrowth. Nature Neurosci 5:431-
Moore RY, Bernstein M (1989) Synaptogenesis in the rat suprachiasmatic nucleus demonstrated by electron
microscopy and synapsin I immunoreactivity. J Neurosci 9:2151-2162.
Nayak AS, Moore CI, Browning MD (1996) CaM kinase II
phosphorylation of the presyn
aptic protein synapsin is
persistently increased during expression of long-term po
tentiation. Proc Natl Acad Sci (USA) 93:15451-15456.
Stone LM, Browning MD, Finger TE (1994) Differential dist
ribution of the synapsins in the rat olfactory bulb. J
Kurtis D. Davies, Susan M. Goebel-Goody, St
even J. Coultrap, and Michael D. Browning (2008)
Long Term Synaptic
Depression That Is Associated with GluR1 Dephosphorylation but Not
isoxazolepropionic Acid (AMPA) Receptor Internalization
J. Biol. Chem.,
283: 33138 - 33146.
Note: Dr. Michael Browning, co-author of the
cited papers is the President and founder
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