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Phospho-Thr53 Dopamine Transporter (DAT) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Thr53 Dopamine Transporter (DAT) Antibody AN1234
    Western blot of rat caudate lysate showing specific immunolabeling of the ~ 55kglycosylated form of the DAT protein phosphorylated at Thr53. Immunolabelingis blocked by the phospho-peptide used as antigen (peptide), but not by thecorresponding dephospho-peptide (not shown).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P23977
Reactivity Rat
Host Rabbit
Clonality polyclonal
Calculated MW 55 KDa
Additional Information
Gene Name SLC6A3
Other Names Sodium-dependent dopamine transporter, DA transporter, DAT, Solute carrier family 6 member 3, Slc6a3
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Antibody Specificity Specific for the ~55k glycosylated form of the DAT proteinphosphorylated at Thr53. Relative mobility may vary depending on the state of glycosylation ofthe DAT protein. Immunolabeling of the DAT band is blocked by preadsorption with thephospho-peptide used as antigen but not by the corresponding dephospho-peptide.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Thr53 Dopamine Transporter (DAT) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
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The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996). It has been shown that phosphorylation at Thr53 directly affects dopamine influx and amphetamine-stimulated substrate efflux, indicating that the Thr53 residue plays a major role in transport activity (Foster et al., 2012).


Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock
JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users.
Neuropsychopharmacology 24:561-567.
Wilson JM, Kalasinsky KS, Levey AI, Bergeron C, Reiber G, Anthony RM, Schmunk GA, Shannak K,
Haycock JW, Kish SJ (1996) Striatal dopamine nerve terminal markers in human, chronic
methamphetamine users. Nat Med 2:699-703.
Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the
locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.
Zhu MY, Klimek V, Dilley GE, Haycock JW, StockmeierC, Overholser JC, Meltzer HY, Ordway GA (1999)
Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry
James D. Foster, Jae-Won Yang, Amy E. Moritz, Sathyavathi ChallaSivaKanaka, Margaret A. Smith,
Marion Holy, Kyle Wilebski, Harald H. Sitte, and Roxanne A. Vaughan (2012) Dopamine Transporter
Phosphorylation Site Threonine 53 Regulates Substrate Reuptake and Amphetamine-stimulated Efflux.
Journal of Biological Chemistry 287(35):29702-29712.

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$ 335.00
Cat# AN1234
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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