Phospho-Thr52 Activating Transcription Factor 2 (ATF2) Antibody
Affinity purified rabbit polyclonal antibody
|Calculated MW||74 KDa|
|Other Names||Cyclic AMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding protein 2, HB16, Histone acetyltransferase ATF2, cAMP response element-binding protein CRE-BP1, ATF2, CREB2, CREBP1|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding THr52 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.|
|Antibody Specificity||Specific for the ~72k ATF2 protein phosphorylated at Thr52. Immunolabeling of the ATF2 band is reduced by treatment of the cells with the PKC catalytic inhibitor Gö6850.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Thr52 Activating Transcription Factor 2 (ATF2) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The transcription factor ATF2 is a member of the ATF/CREB family of leucine zipper proteins. In response to stress stimuli, it activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype (Vlahopoulos et al., 2008). Inhibiting ATF2 impedes melanoma development and elicits tumor suppressor function (Bhoumik et al., 2008). To act as a tumor suppressor, ATF2 must localize at the mitochondria, and phosphorylation at Thr52 by PKCε regulates this translocation (Lau et al., 2012).
Vlahopoulos SA, Logotheti S, Mikas D, Giarika A, Gorgoulis V, and Zoumpourlis V. (2008) The role of ATF-2 in oncogenesis. Bioessays Apr;30(4):314-27. Review.
Bhoumik A and Ronai Z. (2008) ATF2: a transcription factor that elicits oncogenic or tumor suppressor activities. Cell Cycle Aug;7(15):2341-5. Epub 2008 Jun17. Review.
Lau E, Kluger H, Varsano T, Lee K, Scheffler I, Rimm D, Ideker T, and Ronai Z. (2012) PKC Promote Oncogenic Functions of ATF2 in the Nucleus while Blocking Its Apoptotic Function at Mitochondria. Cell 148, 543-555.
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