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Phospho-Ser43 Troponin I (cardiac) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Ser43 Troponin I (cardiac) Antibody AN1236
    Western blot of mouse heart lysate showing specific immunolabeling of the ~25k cTnI protein phosphorylated at Ser43 (control). Phosphospecificity is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that the lysate was incubated in λ-Ptase (1400 units for 30 min). The immunolabeling is greatly decreased by treatment with λ-Ptase.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P48787
Reactivity Mouse, Rat
Predicted Bovine, Human, Monkey, Xenopus, Zebrafish
Host Rabbit
Clonality polyclonal
Calculated MW 25 KDa
Additional Information
Gene ID 21954
Gene Name TNNI3
Other Names Troponin I, cardiac muscle, Cardiac troponin I, Tnni3
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser43 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Antibody Specificity Specific for the ~25k cardiac troponin I protein phosphorylated at Ser43. Immunolabeling is greatly decreased with lambda-phosphatase treatment.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser43 Troponin I (cardiac) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
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Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle (Noland et al, 1995). Protein kinase C-mediated phosphorylation of cardiac myofilament proteins has been shown to depress the actomyosin interaction and may be important during heart failure. (Montgomery et al, 2002) Studies indicate that phosphorylation of Ser43 and Ser45 of cardiac troponin I plays a substantial role in the PKC-mediated depression. (Montgomery et al, 2002).


David E. Montgomery, Beata M. Wolska, W. Glen Pyle, Brian B. Roman, Jasmine C. Dowell, Peter M. buttrick, Alan P. Koretsky, Pedro Del Nido, R. John Solaro (2002) α-Adrenergic response and myofilament activity in mouse hearts lacking PKC phosphorylation sites on cardiac TnI. Am J Physiol Heart Circ 282:H2397-H2405.
Thomas A. Noland, Jr., Xiaodu Guo, Robert L. Raynor, Nathan M. Jideama, Vera Averyhart-Fullard, R. John Solaro, and J.F. Kuo (1995) Cardiac Troponin I Mutants. J of Biol Chem 270 (43): 25445-25454.

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$ 335.00
Cat# AN1236
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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