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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   Phospho-Ser421 Methyl-CpG Binding Protein 2 (MeCP2) Antibody   

Phospho-Ser421 Methyl-CpG Binding Protein 2 (MeCP2) Antibody

Rabbit polyclonal antibody

  • WB - Phospho-Ser421 Methyl-CpG Binding Protein 2 (MeCP2) Antibody AN1238
    Western blot of mouse whole brain showing specific immunolabelingof the ~55k truncated MeCP2 protein phosphorylated at Ser421. Immunolabelingis greatly reduced by preadsorption with the phospho-peptide used as antigen (peptide),but not by the corresponding dephospho-peptide (not shown).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Z2D6
Reactivity Mouse
Predicted Human, Monkey
Host Rabbit
Clonality polyclonal
Calculated MW 52307 Da
Additional Information
Gene ID 17257
Gene Name MECP2
Other Names Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, Mecp2
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.
Antibody Specificity Specific for the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling of the MeCP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser421 Methyl-CpG Binding Protein 2 (MeCP2) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
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MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser421. Phosphorylation and dephosphorylation of this site may be involved in regulation of behavioral responses to chronic antidepressant treatment (Hutchinson et al., 2012).


Amir RE, Van den Veyver IB, Wan M, Tran CQ, Francke U, Zoghbi HY (1999) Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2. Nat Genet. Oct;23(2):185-8.
Hutchinson AN, Deng JV, Cohen S, West AE (2012) Phosphorylation of MeCP2 at Ser421 contributes to chronic antidepressant action. Journal of Neuroscience. Oct 10;32(41):14355-63.
Nan X, Ng HH, Johnson CA, Laherty CD, Turner BM, Eisenman RN, Bird A (1998) Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex. Nature. May 28;393(6683):386-9.

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$ 335.00
Cat# AN1238
(40 western blots)
Availability: 7-10 days
Bulk Size
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