|Predicted||Bovine, Chicken, Human, Pig, Monkey, Rat|
|Calculated MW||55364 Da|
|Other Names||Histone deacetylase 2, HD2, HDAC2|
|Target/Specificity||Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser394 of human HDAC2.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on dephosphopeptide and phosphopeptide affinity columns.|
|Antibody Specificity||Specific for the ~55k HDAC2 protein phosphorylated at Ser394 inWestern blots. Immunolabeling is completely blocked by λ-Phosphatase treatment (30 minutes,800units/1mg protein).|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Ser394 HDAC2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Histone Deacetylase 2 (HDAC2) is part of a family of histone deacetylases that are responsible for deacetylation of lysine residues in the histone core. HDAC2 is classified as a class I histone deacetylase and is ubiquitously expressed throughout the body (Kee et al, 2008). It has been shown that HDAC2 plays an important role in cardiac hypertrophy (Eom et al, 2011). Phosphorylation of ser394 is responsible for the hypertrophy-associated activation of HDAC2, whereas intrinsic basal activity is maintained by phosphorylation of ser422 and ser424 (EOM et al, 2011).
Kee HJ, Eom GH, Joung H, Shin S, Kim JR Cho YK, Choe N, Sim BW, Jo D, Jeong MH, Kim KK, Seo JS,
Kook H (2008) Activation of histone deacetylase 2 by inducible heat shock protein 70 in cardiac
hypertrophy. Cir Res 2008 Nov 21;103(11):1259-69.
Eom GH, Cho YK, Ko JH, Shin S, Choe N, Kim Y, Joung H, Kim HS, Nam KI, Kee HJ, Kook H (2011)
Casein Kinase-2α1 Induces Hypertrophic Respose by Phosphorylation of Histone Deacetylase 2
S394 and its activation in the Heart Clinical Perspective. Circulation May16, 2011;123:2392-2403.
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