|Predicted||Bovine, Chicken, Human, Monkey, Rat|
|Calculated MW||65 KDa|
|Other Names||Syntaxin-binding protein 1, Protein unc-18 homolog 1, Unc18-1, Protein unc-18 homolog A, Unc-18A, Stxbp1|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser241 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.|
|Antibody Specificity||Specific for the ~65k Munc18-1 protein phosphorylated at Ser241. Immunolabeling is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein).|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Ser241 Munc18-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc18-1 is thought to be regulated by phosphorylation.
Ciufo LF, Barclay JW, Burgoyne RD, Morgan A (2005) Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions. Mol Biol Cell 16:470-482.
Liu J, Ernst SA, Gladycheva SE, Lee YY, Lentz SI, Ho CS, Li Q, Stuenkel EL (2004) Fluorescence resonance energy transfer reports properties of syntaxin1a interaction with Munc18-1 in vivo. J Biol Chem 279:55924-55936.
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