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Phospho-Thr84 PAK1 Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Thr84 PAK1 Antibody AN1253
    Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68k PAK1 protein phosphorylated at Thr84 (control). Phosphospecificity is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that the lysate was incubated in λ-Ptase (800 units/1mg proteinfor30min).Theimmunolabelingiscompletelyeliminatedbytreatmentwithλ-Ptase.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13153
Reactivity Human, Mouse
Predicted Bovine, Chicken, Monkey, Rat, Zebrafish
Host Rabbit
Clonality polyclonal
Calculated MW 68 KDa
Additional Information
Gene ID 5058
Gene Name PAK1
Other Names Serine/threonine-protein kinase PAK 1, Alpha-PAK, p21-activated kinase 1, PAK-1, p65-PAK, PAK1
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr84 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Antibody Specificity Specific for the ~68k PAK1 phosphorylated at Thr84.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Thr84 PAK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
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PAKs, p21 activated kinases, are a family of serine/threonine protein kinases comprised of six isoforms, PAK1-6, and they play important roles in cytoskeleton dynamics, cell survival and proliferation (Ye et al, 2012). Each of these isoforms contains a C-terminal catalytic domain and an N-terminal regulatory domain with a small G protein binding motif (Chen et al, 2004). OSR1, oxidative stress response 1, is activated only by osmotic stresses, like sorbital or NaCl (Chen et al, 2004). It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at thr84 and inhibits activation of JNK and MAPK pathway. (Chen et al, 2004). It has also been suggested that OSR1 may have a regulating function with actin cytoskeleton because it can phosphorylate PAK1 at thr84 and bind to gelsolin (Chen et al, 2004).


Chen W, Yazicioglu M, Cobb MH (2004) Characterization of OSR1, a member of the
mammalian Ste20p/germinal center kinase family. J of Biol Chem 279 (12):11129-36.
Ye D, Field J (2012) PAK signaling in cancer. Cell Logist 2(2):105-116.

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$ 335.00
Cat# AN1253
(40 western blots)
Availability: 7-10 days
Bulk Size
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