|Calculated MW||34 KDa|
|Other Names||DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, Protein regulated in development and DNA damage response 1, REDD-1, DDIT4, REDD1, RTP801|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr23/25 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.|
|Antibody Specificity||Specific for the ~34k REDD1 phosphorylated at Thr23/25. Immunolabelingis blocked by the phosphopeptide used as antigen but not by the correspondingdephosphopeptide.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Thr23/25 REDD1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD 1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia c an desensitize cells to apoptotic stimuli (Schwarzer et al, 2005). REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress (Katiyar et al, 2009). It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A–DDB1–ROC1–b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr 25, Thr 23 and Ser 19 throu gh the activity of GSK3b (Katiyar et al, 2009).
Schwarzer r, Tondera D, Arnold W, Giese K, Klippel A, Kaufmann J (2005) REDD1 integrates
hypoxia-mediated survival signaling downstream
of phosphatidylinositol 3-kinase. Oncogene
Katiyar S, Liu E, Knutzen CA, Lang ES, Lombardo CR, Sankar S, Toth JI, Petroski MD, Ronai
Z, Chiang GC (2009) REDD1, an inhibitor of mTOR signaling, is regulated by the CUL4A-
DDB1 ubiquitin ligase. EMBO Rep 10(8):866-72.
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