|Application ||WB, E|
|Clone Names||4D2D9G8, 4A10|
|Description||Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.|
|Immunogen||Human IgG was isolated from human sera and purified by chromatography.|
|Formulation||Purified antibody in PBS containing 0.03% sodium azide.|
|Dilution||WB~~1/500 - 1/2000|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||human IgG Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Roitt,I, et al"Immunology", Mosby, London, England, (1996) fourth edition.2. Vlug, A. et al"The structure and function of human IgG subclasses". Eur.Clin.Lab. 8, 26 (1989).
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