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human IgG Antibody

Purified Mouse Monoclonal Antibody

  • WB - human IgG Antibody AO1002a
    Figure 1: Western blot analysis using IgG mouse mAb (lane 1, 2) and Albumin mouse mAb (lane 3, 4) against human serum (lane 1, 3) and plasma (lane 2, 4).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4D2D9G8, 4A10
Isotype IgG1
Calculated MW 50kDa
Description Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
Immunogen Human IgG was isolated from human sera and purified by chromatography.
Formulation Purified antibody in PBS containing 0.03% sodium azide.
Additional Information
Dilution WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionshuman IgG Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)

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1. Roitt,I, et al"Immunology", Mosby, London, England, (1996) fourth edition.2. Vlug, A. et al"The structure and function of human IgG subclasses". Eur.Clin.Lab. 8, 26 (1989).

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$ 300.00
Cat# AO1002a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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