|Application ||WB, IHC, E|
|Calculated MW||43327 Da|
|Description||Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions|
|Immunogen||Purified recombinant fragment of KSHV ORF45 expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Protein ORF45, ORF45|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KSHV ORF45 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Prevents the establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of host interferon regulatory factor 7/IRF7, a transcription factor critical for the induction of interferons alpha and beta. Mechanistically, ORF45 competes with the associated IRF7 and inhibits its phosphorylation by IKBKE or TBK1 by acting as an alternative substrate.|
|Cellular Location||Virion tegument. Host cytoplasm. Host nucleus|
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Provided below are standard protocols that you may find useful for product applications.
1. Chang Y. et al. 1994. Science. 266:1865-1869. 2. Zhu FX. et al. 2002. PNAS. 99: 5573-5578. 3. Zhu FX. et al. J Virol. 2003. April (77): 4221-4230.
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