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KSHV ORF45 Antibody
Purified Mouse Monoclonal Antibody
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- CITATIONS
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- BACKGROUND
Application 
| WB, IHC, E |
|---|---|
| Primary Accession | F5HDE4 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 2D4A5 |
| Isotype | IgG1 |
| Calculated MW | 43327 Da |
| Description | Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions |
| Immunogen | Purified recombinant fragment of KSHV ORF45 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Gene ID | 4961474 |
|---|---|
| Other Names | Protein ORF45, ORF45 |
| Dilution | WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | KSHV ORF45 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ORF45 |
|---|---|
| Function | Prevents the establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of host interferon regulatory factor 7/IRF7, a transcription factor critical for the induction of interferons alpha and beta (PubMed:11943871, PubMed:20485504, PubMed:22787218). Mechanistically, ORF45 competes with the associated IRF7 and inhibits its phosphorylation by IKBKE or TBK1 by acting as an alternative substrate (PubMed:11943871, PubMed:22787218). Acts as an activator of the NLRP1 inflammasome via interaction with the N-terminal part of host NLRP1: interaction promotes translocation of the N-terminal part of NLRP1 into the nucleus, relieving autoinhibition of the NLRP1 inflammasome and leading to its activation (PubMed:35618833). Also plays a role in promoting the late transcription and translation of viral lytic genes by constitutively activating host extracellular signal-regulated kinase (ERK)-p90 ribosomal S6 kinase/RPS6KA1 (PubMed:30842327). In addition, supports the viral replication cycle by modulating host p53/TP53 signaling pathway (PubMed:34523970). Interacts with host p53/TP53 and prevents its interaction with the deubiquitinase USP7, leading to sequestration of P53/TP53 in the host cytoplasm thereby diminishing its transcriptional activity (PubMed:34523970). |
| Cellular Location | Virion tegument. Host cytoplasm. Host nucleus. Host Golgi apparatus |
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1. Chang Y. et al. 1994. Science. 266:1865-1869. 2. Zhu FX. et al. 2002. PNAS. 99: 5573-5578. 3. Zhu FX. et al. J Virol. 2003. April (77): 4221-4230.
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$ 385.00
Cat# AO1016a
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