|Application ||WB, IHC, E|
|Calculated MW||36336 Da|
|Description||Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. At least five major proteins are likely to be involved in the assembly of the HHV-8 capsid, including a protease (encoded by ORF17), the major capsid protein (encoded by ORF25), and three other smaller capsid proteins (encoded by ORF62, ORF26, and ORF65). Previous structural studies have shown that the HSV-1 triplex is a monomer of VP19c and a dimer of VP23 and that the HCMV triplex is similarly composed of a monomer and a dimer. By analogy, the KSHV triplexes are likely also composed of a monomer of the ORF62 protein and a dimer of the ORF26 protein, which are the respective homologs of VP19c and VP23.|
|Immunogen||Purified recombinant fragment of human KSHV ORF62 expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Triplex capsid protein VP19C homolog, ORF62|
|Dilution||IHC~~1/200 - 1/1000|
WB~~1/500 - 1/2000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KSHV ORF62 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Structural component of the T=16 icosahedral capsid. The capsid is composed of pentamers and hexamers of the major capsid protein ORF25, which are linked together by heterotrimers called triplex. These triplex are formed by a single molecule of VP19C and two copies of VP23 which bridge major capsid protein ORF25 multimers together. Triplexes occupy the local threeflod positions between hexons and between hexamers and pentamers. Additionally, VP19C is required for efficient transport of VP23 to the nucleus, which is the site of capsid assembly.|
|Cellular Location||Virion. Host nucleus.|
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Provided below are standard protocols that you may find useful for product applications.
1. Chang Y. et al. 1994. Science. 266:1865-1869. 2. Russo J.J. et al. 1996. PNAS. 93: 14862-14867. 3. Wu L. et al. 2000. J VirolOct; 74(20):9646-54.
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