GST Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Host | Mouse |
Clonality | Monoclonal |
Clone Names | 6G9C6; 6G9B9 |
Isotype | IgG1 |
Description | Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. Expression vectors with Glutathione S-transferase (GST) tag have beed developed widely for both prokaryotic and eukaryotic systems, because GST can provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein encoded by inserted cDNA sequences. Antibodies of anti-GST tag are useful for checking protein expression using immunoblotting, immunoprecipitation and immunostaining techniques, as well as for immunoaffinity purification of protein expressed in vitro. |
Immunogen | Purified fusion protein with GST protein tag expressed in E. Coli. |
Formulation | Purified antibody in PBS containing 0.03% sodium azide. |
Dilution | WB~~1/500 - 1/2000 |
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Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | GST Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
References
1. 1993. Biotechnology (N Y) 11: 933-6. 2. 1988. Gene 67: 31-40.
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