|Application ||WB, ICC, E|
|Clone Names||2G1H11; 1F1E8|
|Calculated MW||41213 Da|
|Description||Apolipoprotein A5 (ApoA5) is fast gaining attention as a key regulator of serum triglyceride concentrations. An ApoA5 mouse knock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model with human ApoA5 produced 50–70% lower concentrations of mouse serum triglycerides. In addition, peroxisome proliferator-activated receptor- agonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. Despite these compelling molecular biology data, relatively little is known about ApoA5 protein in human serum.This antibody pair detected recombinant apoa5 protein in sandwich ELISA format and could be potential reagents for the development of clinical diagnostic kits.|
|Immunogen||Purified recombinant fragment of human Apoa5 expressed in E. Coli. |
|Formulation||Purified antibody in PBS containing 0.03% sodium azide.|
|Other Names||Apolipoprotein A-V, Apo-AV, ApoA-V, Apolipoprotein A5, Regeneration-associated protein 3, APOA5, RAP3|
|Dilution||IHC~~1/500 - 1/2000|
WB~~1/500 - 1/2000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||APOA5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages.|
|Tissue Location||Liver and plasma.|
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Provided below are standard protocols that you may find useful for product applications.
1．Pennacchio, L， Science 2001. 294, 169-173. 2．Prieur, X. J Biol Chem 2003. 278, 25468-25480.
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