|Application ||WB, E|
|Calculated MW||60183 Da|
|Description||The TUG protein contains a UBX domain, for GLUT4. In truncated form, TUG acts in a dominant-negative manner to inhibit insulin-stimulated GLUT4 redistribution in Chinese hamster ovary cells and 3T3-L1 adipocytes. Full-length TUG forms a complex specifically with GLUT4. In 3T3-L1 adipocytes, this complex is present in unstimulated cells and is largely disassembled by insulin. Endogenous TUG is localized with the insulin-mobilizable pool of GLUT4 in unstimulated 3T3-L1 adipocytes, and is not mobilized to the plasma membrane by insulin.|
|Immunogen||Purified recombinant fragment of TUG expressed in E. Coli.|
|Formulation||Purified antibody in PBS containing 0.03% sodium azide.|
|Other Names||Tether containing UBX domain for GLUT4, Alveolar soft part sarcoma chromosomal region candidate gene 1 protein, Alveolar soft part sarcoma locus, Renal papillary cell carcinoma protein 17, UBX domain-containing protein 9, ASPSCR1, ASPL, RCC17, TUG, UBXD9, UBXN9|
|Dilution||WB~~1/500 - 1/2000|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mouse TUG Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ASPL, RCC17, TUG, UBXD9, UBXN9|
|Function||Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface (By similarity). Enhances VCP methylation catalyzed by VCPKMT.|
|Cellular Location||Endomembrane system; Peripheral membrane protein. Endoplasmic reticulum- Golgi intermediate compartment membrane; Peripheral membrane protein Cytoplasm. Nucleus|
|Tissue Location||Ubiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas.|
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1. Nature 2003,425:727 - 733.
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