- CITATIONS: 1
|Application ||WB, IHC, E|
|Clone Names||3A10A12; 5C3A7|
|Calculated MW||24008 Da|
|Description||cTnI has an apparent molecular weight of 22.5 kDa. cTnI is a candidate marker with acceptable sensitivity and specificity for AMI and other cardiac diseases. Troponin, a molecule that binds to the thin filament (actin) of striated muscle fibers, acts with intracellular calcium to control the interaction of the thin filament with the thick filament (myosin), thus regulating muscle contraction. Troponin I prevents muscle contraction in the absence of calcium, which has two skeletal muscle isoforms with considerable amino acid sequence homology. cTnI contains an additional N-terminal sequence and is highly specific for myocardium.|
|Immunogen||Purified recombinant fragment of cTnI expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Troponin I, cardiac muscle, Cardiac troponin I, TNNI3, TNNC1|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||cTnI Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.|
Provided below are standard protocols that you may find useful for product applications.
1. Cummins B and Cummins P, J Mol Cell Cardiol, 1987, 19(10):999-1010. 2. Cummins B, Auckland ML, and Cummins P, Am Heart J, 1987, 113(6):1333-44. 3. Darnell J, Lodish H, and Baltimore D, Molecular Cell Biology, New York, NY: Scientific American Books, 1986, 827-8. 4. Larue C, Defacque-Lacquemant H, Calzolari C, et al, Mol Immunol, 1992, 29(2):271-8. 5. Adams JE III, Bodor GS, Davila-Roman VG, et al, Circulation, 1993, 88(1):101-6.
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