|Application ||WB, IHC, E|
|Calculated MW||26210 Da|
|Description||Calcyclin encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma.|
|Immunogen||Purified recombinant fragment of calcyclin expressed in E. Coli.|
|Formulation||Purified antibody in PBS containing 0.03% sodium azide.|
|Other Names||Calcyclin-binding protein, CacyBP, hCacyBP, S100A6-binding protein, Siah-interacting protein, CACYBP, S100A6BP, SIP|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Calcyclin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).|
|Cellular Location||Nucleus. Cytoplasm. Note=Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated|
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1. Bean C.et.al J Mol Biol. 2005 Jun 3;349(2):349-66. 2. HWang R.et.al J Biol Chem. 2004 May 14;279(20):21239-47.
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