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NPT Antibody

Purified Mouse Monoclonal Antibody

  • WB - NPT Antibody AO1047a
    Figure 1: Western blot analysis using NPT mouse mAb against truncated NPT recombinant protein.
  • IHC - NPT Antibody AO1047a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human ovary carcinoma (left) and breast carcinoma (right), showing cytoplasmic localization using EphA1 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4B4D1
Isotype IgG2a
Description Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.
Immunogen Purified recombinant fragment of NPT expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Dilution WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNPT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
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1. Landis WG Human and ecological Risk Assessment 6(5): 875-899. 2. Stewart CN BioTechniques 29(4): 832-843. 3. Thomson JA Journal of Food Science 66(2): 188-193.

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$ 300.00
Cat# AO1047a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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