- CITATIONS: 1
|Application ||WB, E|
|Description||Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.|
|Immunogen||Purified recombinant fragment of NPT expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Dilution||WB~~1/500 - 1/2000|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NPT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Provided below are standard protocols that you may find useful for product applications.
1. Landis WG et.al Human and ecological Risk Assessment 6(5): 875-899. 2. Stewart CN et.al BioTechniques 29(4): 832-843. 3. Thomson JA et.al Journal of Food Science 66(2): 188-193.
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