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FGF2 Antibody

Purified Mouse Monoclonal Antibody

  • WB - FGF2 Antibody AO1048a
    Figure 1: Western blot analysis using FGF2 mouse mAb against truncated FGF2 recombinant protein.
  • IHC - FGF2 Antibody AO1048a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human recturn adenocarcinoma tissue showing cytoplasmic localization using FGF2 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09038
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2H5G2C11
Calculated MW 30770 Da
Description FGF2 is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of anguogenesis. Due to its basic pH, the factor is named FGF-2 (basic FGF, bFGF).Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).
Immunogen Purified recombinant fragment of FGF2 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 2247
Other Names Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2, HBGF-2, FGF2, FGFB
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFGF2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FGF2
Synonyms FGFB
Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
Cellular Location Secreted. Nucleus. Note=Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57
Tissue Location Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue.
Research Areas
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1. Romanov VV Oncogene. 2005,Oct 13; 24(45) : 6855-60. 2. Webber CA Mol Cell Neurosci. 2005, Sep; 30 (1):37-47.

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$ 300.00
Cat# AO1048a
(40 western blots)
Availability: 7-10 days
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