|Application ||WB, E|
|Calculated MW||141466 Da|
|Description||Glucagon-like peptide-1 (GLP-1) is an incretin hormone secreted from enteroendocrine L cells in response to ingested nutrients. The closely related peptides glucagon-like peptide (GLP-1) and glucagon have opposing effects on blood glucose. GLP-1 induces glucose-dependent insulin secretion in the pancreas, while glucagon stimulates gluconeogenesis and glycogenolysis in the liver. Glucagon is processed from a large precursor, proglucagon, in a tissue-specific manner in pancreatic alpha-cells. The identification of a hybrid peptide acting as both a GLP-1 agonist and a glucagon antagonist would provide a novel approach for the treatment of type 2 diabetes.|
|Immunogen||Purified recombinant fragment of GLP expressed in E. Coli.|
|Formulation||Purified antibody in PBS containing 0.03% sodium azide.|
|Other Names||Histone-lysine N-methyltransferase EHMT1, 2.1.1.-, 126.96.36.199, Euchromatic histone-lysine N-methyltransferase 1, Eu-HMTase1, G9a-like protein 1, GLP, GLP1, Histone H3-K9 methyltransferase 5, H3-K9-HMTase 5, Lysine N-methyltransferase 1D, EHMT1, EUHMTASE1, GLP, KIAA1876, KMT1D|
|Dilution||WB~~1/500 - 1/2000|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GLP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||EUHMTASE1, GLP, KIAA1876, KMT1D|
|Function||Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non- histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.|
|Cellular Location||Nucleus. Chromosome. Note=Associates with euchromatic regions|
|Tissue Location||Widely expressed.|
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1. Clark Q. Pan, Joanne M. Buxton, Stephanie L. Yung, et al. J Biol Chem. 2006 Feb 27. 2.Michael F. Crutchlow, Jee-Young Nina Ham, et al. Int J Biochem Cell Biol. 2006;38(5-6):845-859. 3.Andrew Young Adv Pharmacol. 2005;52:151-71.
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