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EphB6 Antibody

Purified Mouse Monoclonal Antibody

  • WB - EphB6 Antibody AO1051a
    Figure 1: Western blot analysis of Jurkat (1) and NIH/3T3 (2) cell lysate using EphB6 mouse mAb.
  • IHC - EphB6 Antibody AO1051a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human bladder carcinoma (left) and return carcinoma (right) tissue, showing cytoplasmic localization using EphB6 mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O15197
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8E7H12
Isotype IgG1
Calculated MW 110700 Da
Description EPH receptor B6 (EphB6), with 1006-amino acid protein(~ 110 kDa), belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Eph receptors and ephrin ligands are membrane-bound cell-cell communication molecules with well-defined functions in development. EphB6 is expressed both in a variety of embryonic and adult tissues. EphB6 is a unique member in the Eph family of receptor tyrosine kinases in that its kinase domain contains several alterations in conserved amino acids and is catalytically inactive. EphB6 can both positively and negatively regulate cell adhesion and migration and tyrosine phosphorylation of the receptor by an Src family kinase acts as the molecular switch for the functional transition.
Immunogen Purified recombinant fragment of EphB6 expressed in E. Coli.
Formulation Purified antibody in PBS containing 0.03% sodium azide.
Additional Information
Gene ID 2051
Other Names Ephrin type-B receptor 6, HEP, Tyrosine-protein kinase-defective receptor EPH-6, EPHB6
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEphB6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPHB6
Function Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed in brain. Expressed in non invasive breast carcinoma cell lines (at protein level). Strong expression in brain and pancreas, and weak expression in other tissues, such as heart, placenta, lung, liver, skeletal muscle and kidney Expressed in breast non invasive tumors but not in metastatic lesions. Isoform 3 is expressed in cell lines of glioblastomas, anaplastic astrocytomas, gliosarcomas and astrocytomas. Isoform 3 is not detected in normal tissues.
Citations (0)

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1. Kazushige Ogawa, Hiroki Wada, Noriyoshi Okada J Cell Sci. 2006 Feb 1;119(Pt 3):559-70. 2. Hiroshi Matsuoka, Hiroya Obama, Meghan L. Kelly J Biol Chem. 2005 Aug 12;280(32):29355-63.

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$ 385.00
Cat# AO1051a
(40 western blots)
Availability: 7-10 days
Bulk Size
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