|Application ||WB, IHC, E|
|Calculated MW||25673 Da|
|Description||Steroid receptor RNA activator 1 (SRA), with 237-amino acid protein (about 27kDa), belongs to the growing family of functional non-coding RNAs. SRA was originally described as the first functional noncoding RNA able to specifically coactivate the activity of steroid receptors. Specifically, SRA exists as both an RNA transcript that forms a complex with steroid receptor coactivator-1 and as a stably expressed protein. Its expression is strongly up-regulated in many human tumors of the breast, uterus, and ovary, suggesting a potential role in pathogenesis. Although coactivation of steroid-dependent transcription by SRA is accompanied by a proliferative response, overexpression is not in itself sufficient to induce turmorigenesis.|
|Immunogen||Purified recombinant fragment of SRA expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Steroid receptor RNA activator 1, Steroid receptor RNA activator protein, SRAP, SRA1 (HGNC:11281)|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SRA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand- dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis.|
|Cellular Location||Nucleus. Cytoplasm|
|Tissue Location||Highly expressed in liver and skeletal muscle and to a lesser extent in brain. Also expressed in both normal and tumorigenic breast epithelial cell lines. Significantly up- regulated in human tumors of the breast, ovary, and uterus|
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Provided below are standard protocols that you may find useful for product applications.
1. Rainer B. Lanz, Steven S. Chua, Niall Barron. Mol. Cell. Biol, Oct 2003; 23: 7163 - 7176. 2. Shilpa Chooniedass-Kothari, Mohammad Kariminia Hamedani, Sandy Troup. Int J Cancer. 2006 Feb 15;118(4):1054-9 3. S. Chooniedass-Kothari, E. Emberley, M. K. Hamedani. FEBS Lett. 2004 May 21;566(1-3):43-7.
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