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ZAP70 AntibodyMouse Monoclonal Antibody to ZAP70
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AO1058a | 0.1ml 400 ul | 7-10 days | $ 385.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ZAP70 Antibody - Product info | |
| Application | WB, IHC
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| Primary Accession | P43403 |
| Other Accession | NP_997402.1 |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Clone Names | 3D2A4 |
| Calculated MW | 69872 Da |
ZAP70 Antibody - Additional info | |
| Gene ID 7535 | |
| Other Names SRK; STD; TZK | |
| Target/Specificity Purified recombinant fragment of human ZAP70 expressed in E. Coli. | |
| Dilution WB~~1:500 - 2000. IHC~~1:200 - 1000. | |
| Format Purified recombinant fragment of human ZAP70 expressed in E. Coli. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ZAP70 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
ZAP70 Antibody - Protein Information | |
| Name ZAP70 | |
| Synonyms SRK | |
| Function Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2 Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR) | |
| Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein. Note=In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity) | |
| Tissue Location Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells | |
ZAP70 Antibody - Related products
AP3676a: Phospho-ZAP70-Y292 Antibody
AP7824c: ZAP70 Antibody (Center)
RI15782: ZAP70 predesign siRNA
BP3658a: Phospho-ZAP70-Y319 Blocking Peptide
BP3659a: Phospho-ZAP70-Y492 Blocking Peptide
BP3660a: Phospho-ZAP70-Y493 Blocking Peptide
BP3661a: Phospho-ErbB2-pY1248(M) Blocking Peptide
BP3676a: Phospho-ZAP70-Y292 Antibody Blocking Peptide
BP7824c: ZAP70 Antibody (Center) Blocking Peptide
AJ1824b: ZAP-70 Antibody (C-term)
ZAP70 Antibody - Application data
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Figure 1: Western blot analysis using anti-ZAP70 mAb against Jurkat cell lysate (1).
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Figure 2: Immunohistochemical analysis of paraffin-embedded human lymph tumor, showing cytoplasmic localization using anti-ZAP70 mAb with DAB staining
ZAP70 Antibody - Research Areas
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BACKGROUND
ZAP70 (zeta-chain associated protein kinase), a 70 kDa member of the SYK tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. ZAP70 controls TCR(T-cell antigen receptor)-linked signal transduction pathways. Its key role in thymocytes development and mature T lymphocytes activation has been illustrated by the characterization of several human immunodeficiencies presenting with mutations in the ZAP70 gene. ZAP70 is also expressed in several types of B-cell neoplasm and is easily detected by immunohistochemistry, providing a useful prognostic marker in patients with chronic lymphocytic leukaemia.
REFERENCES
1. Sigal Gelkop , Gerrald D. Gish, Yael Babichev J Immunol. 2005 Dec 15;175(12):8123-32.
2. Joaquim Carreras , Neus Villamor ,Lluis Colomo J Pathol. 2005 Mar;205(4):507-13.
3. Claire Hivroz Med Sci (Paris). 2005 Feb;21(2):150-5.
