|Application ||WB, IHC, E|
|Clone Names||6G2F1; 6G2F2|
|Calculated MW||50981 Da|
|Description||GSK3 alpha (Glycogen synthase kinase 3 alpha), with 483-amino acid protein (about 53kDa), belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily,GSK3 subsubfamily, which also includes GSK3 beta.GSK3 is a multifunctional serine/threonine kinase that is usually inactivated by serine phosphorylation in response to extracellular cues. GSK3 is a key regulator of numerous signalling pathways, including cellular responses to Wnt, receptor tyrosine kinases and G-protein-coupled receptors and is involved in a wide range of cellular processes, ranging from glycogen metabolism to cell cycle regulation and proliferation.GSK3 alpha is implicated in the control of several regulatory proteins including glycogen synthase and transcription factors.|
|Immunogen||Purified recombinant fragment of GSK3 alpha expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Glycogen synthase kinase-3 alpha, GSK-3 alpha, 22.214.171.124, Serine/threonine-protein kinase GSK3A, 126.96.36.199, GSK3A|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GSK3 alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation.|
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1. Bradley W. Doble and James R. Woodgett. J. Cell Sci., Apr 2003; 116: 1175. 2. C. Laura Sayas , Aafke Ariaens, Bas Ponsioen. Mol. Biol. Cell, Apr 2006; 17: 1834 – 1844.
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