|Application ||WB, IHC, E|
|Calculated MW||53536 Da|
|Description||Desmin (DES), with 470-amino acid protein (about 52kDa), belongs to the intermediate filament family and Desmin is class III intermediate filaments found in muscle cells. Homopolymers of Desmin form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane.Mutations in Desmin are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.Desmin is also expressed in smooth muscle cells of both airways and alveolar ducts and Desmin is a load-bearing protein that stiffens the airways and consequently the lung and modulates airway contractile response.|
|Immunogen||Purified recombinant fragment of Desmin expressed in E. Coli.|
|Formulation||Purified antibody in PBS containing 0.03% sodium azide.|
|Other Names||Desmin, DES|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Desmin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction.|
|Cellular Location||Cytoplasm, myofibril, sarcomere, Z line Cytoplasm. Cell membrane, sarcolemma. Note=Localizes in the intercalated disks which occur at the Z line of cardiomyocytes (PubMed:24200904, PubMed:26724190).|
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Provided below are standard protocols that you may find useful for product applications.
1. Felix R. Shardonofsky, Yassemi Capetanaki, and Aladin M. Boriek. Am J Physiol Lung Cell Mol Physiol, May 2006; 290: L890 - L896. 2. Xupei Huang, Jian Li, Dalton Foster. Experimental Biology and Medicine, Dec 2002; 227: 1039.
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