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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GSTP1 Antibody   

GSTP1 Antibody

Purified Mouse Monoclonal Antibody

  • WB - GSTP1 Antibody AO1078a
    Figure 1: Western blot analysis using GSTP1 mouse mAb against PC3 cell lysate (1) and human cerebellum tissue lysate (2).
  • WB - GSTP1 Antibody AO1078a
    Figure 2: Western blot analysis using GSTP1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GSTP1 cDNA (2).
  • IHC - GSTP1 Antibody AO1078a
    Figure 3: Immunohistochemical analysis of paraffin-embedded human prostate tissues using GSTP1 mouse mAb with DAB staining.
  • IF - GSTP1 Antibody AO1078a
    Figure 4: Confocal immunofluorescence analysis of HepG2 (left) and L-02 (right) cells using GSTP1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • IF - GSTP1 Antibody AO1078a
    Figure 5: Confocal immunofluorescence analysis of PC-3 cells using GSTP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • FC - GSTP1 Antibody AO1078a
    Figure 6: Flow cytometric analysis of K562 cells using GSTP1 mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09211
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3F2C2
Isotype IgG1
Calculated MW 23kDa
Description GSTP1 (glutathione-S-transferase, pi 1), also called GST3/DFN7, is a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 act like a tumor suppressor gene, which when inactivated leads to tumor growth, and the -class glutathione S-transferase is commonly inactivated by somatic CpGisland hypermethylation in cancers of the prostate, liver, and breast. Methylation of regulatory sequences at the GSTP1 gene locus is found in the vast majority (>90%) of prostate carcinomas and is associated with transcriptional down-regulation.
Immunogen Purified recombinant fragment of human GSTP1 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 2950
Other Names Glutathione S-transferase P,, GST class-pi, GSTP1-1, GSTP1, FAEES3, GST3
Dilution WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGSTP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GSTP1
Synonyms FAEES3, GST3
Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.
Cellular Location Cytoplasm. Mitochondrion. Nucleus. Note=The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization
Research Areas
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1. Kimihiko Satoh, Ken Itoh, Masayuki Yamamoto. 2002. Carcinogenesis. 23: 457 - 462.2. Xiaohui Lin,William G. Nelson.2003.Cancer Research. 63: 498-504.

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$ 300.00
Cat# AO1078a
(40 western blots)
Availability: 7-10 days
Bulk Size
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