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4E-BP1 AntibodyMouse Monoclonal Antibody to 4E-BP1
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AO1085b | 0.1ml 400 ul | 7-10 days | $ 385.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
4E-BP1 Antibody - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q13541 |
| Other Accession | NP_004086.1 |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Clone Names | 4B6G10 |
| Calculated MW | 12580 Da |
4E-BP1 Antibody - Additional info | |
| Gene ID 1978 | |
| Other Names BP-1; 4EBP1; 4E-BP1; PHAS-I; MGC4316; EIF4EBP1 | |
| Target/Specificity Purified recombinant fragment of 4E-BP1 expressed in E. Coli. | |
| Dilution WB~~1:500 - 2000. IHC~~1:200 - 1000. | |
| Format Purified recombinant fragment of 4E-BP1 expressed in E. Coli. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions 4E-BP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
4E-BP1 Antibody - Protein Information | |
| Name EIF4EBP1 | |
| Function Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways | |
4E-BP1 Antibody - Related products
AP12627c: EIF4EBP1 Antibody (Center)
AP1981a: EIF4EBP1 Antibody (S100)
AP1981b: EIF4EBP1 Antibody (T36)
AP1981c: EIF4EBP1 Antibody (T69)
AP1981d: EIF4EBP1 Antibody(S111)
AP3353a: Phospho-EIF4EBP1-pS100 Antibody
AP3417a: Phospho-EIF4EBP1-pT36 Antibody
AP3473a: Phospho-EIF4EBP1-S111 Antibody
RI11733: EIF4EBP1 predesign siRNA
LY10995a: EIF4EBP1 Over-expression Lysate
BP1981a: EIF4EBP1 Antibody (S100) Blocking Peptide
BP1981b: EIF4EBP1 Antibody (T36) Blocking Peptide
BP1981c: EIF4EBP1 Antibody (T69) Blocking Peptide
BP3417a: Phospho-EIF4EBP1-pT36 Antibody Blocking Peptide
BP3473a: Phospho-EIF4EBP1-S111 Antibody Blocking Peptide
AJ1004d: 4E-BP1 Antibody Phospho (pT37)
AJ1004e: 4E-BP1 Antibody Phospho (pT70)
AJ1004f: 4E-BP1/2/3 Antibody Phospho (pT45)
4E-BP1 Antibody - Application data
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Figure 1: Western blot analysis using anti-4E-BP1 monoclonal antiobdy against truncated 4E-BP1 recombinant protein(1) and A431 cell lysate(2)
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Figure 2: Immunohistochemical analysis of paraffin-embedded human pancreas carcinoma(A), esophagus carcinoma tissue(B) and ovary tumor tissue(C), showing cytoplasmic and membrane localization using anti-4E-BP1 antibody with DAB staining.
4E-BP1 Antibody - Research Areas
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BACKGROUND
4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.
REFERENCES
1. Pause, A. et al. 1994.Nature. 371:762?767.
2. Fadden, P. et al. 1997. J. Biol. Chem. 272:10240?10247.
