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BRAF Antibody

Purified Mouse Monoclonal Antibody

  • WB - BRAF Antibody AO1086a
    Figure 1: Western blot analysis using BRAF mouse mAb against truncated recombinant Braf (1) and A431 cell lysate (2).
  • WB - BRAF Antibody AO1086a
    Figure 2: Western blot analysis using BRAF mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Braf cDNA (2).
  • IHC - BRAF Antibody AO1086a
    Figure 3: Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue(left) and lung carcinoma tissue (right) showing cytoplasmic localization using BRAF mouse mAb with DAB staining.
  • IHC - BRAF Antibody AO1086a
    Figure 4: Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF mouse mAb.
  • IF - BRAF Antibody AO1086a
    Figure 3: Confocal immunofluorescence analysis of Hela cells using anti-INHA mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15056
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1H12F1; 1H12G10; 1F12F11C9
Isotype IgG1
Calculated MW 84437 Da
Description BRAF(V-raf murine sarcoma viral oncogene homolog B1 ) is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439).B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK),it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers.The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi,primary cutaneous melanomas and colorectal cancers.
Immunogen Purified recombinant fragment of BRAF expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 673
Other Names Serine/threonine-protein kinase B-raf,, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBRAF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms BRAF1, RAFB1
Function Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Cellular Location Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
Tissue Location Brain and testis.
Research Areas
Citations (0)

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1. Rapp, U.R.,et al.1983.Proc. Natl.Acad.Sci.USA.80:4218-4222. 2. Kim J,Giuliano AE,Turner RR.2006.Ann Surg.Nov, 244(5): 799-804. 3. Fullen DR, Poynter JN, Lowe L,2006.Mod Pathol. 19(10): 1324-1332. 4. Terai K, Matsuda M.2006.MBO J.25(15):3556-3564. 5. Noda H,Kato Y,Yoshikawa H,2006.J Exp Clin Cancer Res. 25(2):235-242.

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$ 385.00
Cat# AO1086a
(40 western blots)
Availability: 7-10 days
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