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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Vimentin Antibody   

Vimentin Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - Vimentin Antibody AO1092a
    Figure 1: Western blot analysis using Vimentin mouse mAb against truncated Vimentin recombinant protein.
  • IHC - Vimentin Antibody AO1092a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using Vimentin mouse mAb with DAB staining.
  • ICC - Vimentin Antibody AO1092a
    Figure 2: Immunofluorescence analysis of methanol-fixed L-02 (left) and Cos7 (right) cells using ApoM mouse mAb showing cytoplasmic and membrane localization.
  • IF - Vimentin Antibody AO1092a
    Figure 2: Immunofluorescence analysis of methanol-fixed L-02(left) and COS-7(right) cells using anti-ApoM monoclonal antiobdy showing cytoplasmic and membrane localization.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P08670
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 9E7E7; 5G3F10; 5G3F10E5
Isotype IgG1
Calculated MW 53652 Da
Description Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Immunogen Purified recombinant fragment of Vimentin expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 7431
Other Names Vimentin, VIM
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
ICC~~1:200~~1000
IF~~1:200~1000.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVimentin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines
Research Areas
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References

1. Seshadri, R., et al. Intl. J. Cancer 67: 353-356(1996) 2. Essa, T.M., et al. J. Egyptian Soc. Parasitol. 26:433-442(1996) 3. Chu, Y.W., et al. Amer.J. Pathol. 148: 63-69(1996)

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$ 325.00
Cat# AO1092a
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