|Application ||WB, IHC, E|
|Clone Names||2C8B8; 2C8E8; 3C8D8|
|Calculated MW||10546 Da|
|Description||S100 calcium binding protein A1 (S100-alpha/ S100A1), it is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies.|
|Immunogen||Purified recombinant fragment of S100A1 expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1, S100A1, S100A|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||S100A1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.|
|Tissue Location||Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain|
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Provided below are standard protocols that you may find useful for product applications.
1. Koenig A, Wojcieszyn J, Weeks BR, et al. Vet Pathol. 2001;38(4):427-35. 2. Hoyaux D, Decaestecker C, Heizmann CW, et al. Brain Res. 2000;867(1-2):280-8. 3. Pingerelli PL, Mizukami H, Wagner AS, et al. J Protein Chem. 1990;9(2):169-75.
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