|Application ||WB, IHC, ICC, E|
|Clone Names||4H5E7; 9B10D4|
|Calculated MW||40716 Da|
|Description||CD34 is a transmembrane glycoprotein with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells, endothelial cells and some fibroblasts. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells. CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.|
|Immunogen||Purified recombinant fragment of CD34 expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Hematopoietic progenitor cell antigen CD34, CD34, CD34|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD34 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues|
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Provided below are standard protocols that you may find useful for product applications.
1. Sho E., et al. Arterioscler Thromb Vasc Biol. 2004: 24: 1916-21. 2. Satterthwaite, A.B.,et al. Genomics.1992:12:788-794.3. Krause, D.S.,et al. 1994. Blood.84: 691-701.
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