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CD34 Antibody

Purified Mouse Monoclonal Antibody

  • WB - CD34 Antibody AO1099a
    Figure 1: Western blot analysis using CD34 mouse mAb against truncated CD34 recombinant protein.
  • IHC - CD34 Antibody AO1099a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human spleen using CD34 mouse mAb with DAB staining.
  • IF - CD34 Antibody AO1099a
    Figure 3: Immunofluorescence analysis of peripheral blood cells using CD34 mouse mAb.
  • ICC - CD34 Antibody AO1099a
    Figure 2: Immunofluorescence staining of methanol-fixed Hela cells using ABL2 mouse mAb showing cytoplasm localization.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P28906
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4H5E7; 9B10D4
Isotype IgG2b
Calculated MW 40716 Da
Description CD34 is a transmembrane glycoprotein with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells, endothelial cells and some fibroblasts. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells. CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Immunogen Purified recombinant fragment of CD34 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 947
Other Names Hematopoietic progenitor cell antigen CD34, CD34, CD34
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD34 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD34
Function Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues
Research Areas
Citations (0)

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1. Sho E., et al. Arterioscler Thromb Vasc Biol. 2004: 24: 1916-21. 2. Satterthwaite, A.B.,et al. Genomics.1992:12:788-794.3. Krause, D.S.,et al. 1994. Blood.84: 691-701.

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$ 385.00
Cat# AO1099a
(40 western blots)
Availability: 7-10 days
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