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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Cytokeratin 5 Antibody   

Cytokeratin 5 Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - Cytokeratin 5 Antibody AO1115a
    Figure 1: Western blot analysis using CK5 mouse mAb against truncated CK5 recombinant protein
    detail
  • IHC - Cytokeratin 5 Antibody AO1115a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human esophagus epithelium (A), salivary gland basal cell (B), lung squamous cell carcinoma (C), endometrium admosquamous carcinoma (D), showing cytoplasmic and membrane localization using CK5 mouse mAb with DAB staining.
    detail
  • IHC - Cytokeratin 5 Antibody AO1115a
    Figure 3: Immunohistochemical analysis of paraffin-embedded human skin tissues using CK5 mouse mAb.
    detail
  • IF - Cytokeratin 5 Antibody AO1115a
    Figure 3: Immunofluorescence staining of methanol-fixed Eca-109(left) and HepG2(right) cells showing cytoplasmic localization.
    detail
  • SPECIFICATION
  • CITATIONS: 1
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P13647
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3E2F1
Isotype IgG1
Calculated MW 62378 Da
Description CK5 (keratin 5) is a member of the keratin gene family. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin5 gene (KRT5) have been shown to result in the autosomal dominant disorderepidermolysis bullosa (EB). Defects in KRT5 are a cause of epidermolysis bullosa simplex.
Immunogen Purified recombinant fragment of CK5 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 3852
Other Names Keratin, type II cytoskeletal 5, 58 kDa cytokeratin, Cytokeratin-5, CK-5, Keratin-5, K5, Type-II keratin Kb5, KRT5
Dilution WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
IF~~1:200~1000.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCytokeratin 5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT5
Function Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).
Cellular Location Cytoplasm.
Tissue Location Expressed in corneal epithelium (at protein level) (PubMed:26758872). Expressed in keratinocytes (at protein level) (PubMed:20128788, PubMed:31302245).
Research Areas
Citations ( 0 )

References

1. Canedo-Patzi AM. Leon-Bojorge B. de Ortiz-Hidalgo C. Gac Med Mex. 2006, Jan-Feb, 142(1):59-66. Spanish. 2. Leibl S. Moinfar F. Am J Surg Pathol. 2006, Apr, 30(4):450-6.

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$ 385.00
Cat# AO1115a
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