|Application ||WB, IHC, E|
|Calculated MW||62378 Da|
|Description||CK5 (keratin 5) is a member of the keratin gene family. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin5 gene (KRT5) have been shown to result in the autosomal dominant disorderepidermolysis bullosa (EB). Defects in KRT5 are a cause of epidermolysis bullosa simplex.|
|Immunogen||Purified recombinant fragment of CK5 expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Keratin, type II cytoskeletal 5, 58 kDa cytokeratin, Cytokeratin-5, CK-5, Keratin-5, K5, Type-II keratin Kb5, KRT5|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/500 - 1/2000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Cytokeratin 5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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1. Canedo-Patzi AM. Leon-Bojorge B. de Ortiz-Hidalgo C. Gac Med Mex. 2006, Jan-Feb, 142(1):59-66. Spanish. 2. Leibl S. Moinfar F. Am J Surg Pathol. 2006, Apr, 30(4):450-6.
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