- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
IGF1R-Beta AntibodyMouse Monoclonal Antibody to IGF1R
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AO1121a | 0.1ml 400 ul | 7-10 days | $ 325.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
IGF1R-Beta Antibody - Product info | |
| Application | WB, IHC
|
| Primary Accession | P08069 |
| Other Accession | NP_000866.1 |
| Reactivity | Human |
| Isotype | Mouse IgG2a |
| Clone Names | 3G5C1 |
| Calculated MW | 154793 Da |
IGF1R-Beta Antibody - Additional info | |
| Gene ID 3480 | |
| Other Names IGF1R | |
| Target/Specificity Purified recombinant fragment of IGF1R expressed in E. Coli. | |
| Dilution WB~~1:500 - 2000. IHC~~1:200 - 1000. | |
| Format Purified recombinant fragment of IGF1R expressed in E. Coli. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions IGF1R-Beta Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
IGF1R-Beta Antibody - Protein Information | |
| Name IGF1R | |
| Function Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K- driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R | |
| Cellular Location Membrane; Single-pass type I membrane protein | |
| Tissue Location Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney | |
IGF1R-Beta Antibody - Related products
AP7649a: IGF1 Receptor (IGF1R) Antibody (N-term)
AP7649b: IGF1 Receptor (IGF1R) Antibody (C-term)
AP7649d: IGF1R Antibody (N-term K66)
RI12657: IGF1R predesign siRNA
BP7649a: IGF1R Antibody (N-term) Blocking Peptide
IGF1R-Beta Antibody - Application data
-
Figure 1: Western blot analysis using anti-IGF1R-Beta monoclonal antiobdy against truncated IGF1R recombinant protein.
-
Figure 2: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using IGF1R-Beta mouse mAb with DAB staining.
-
Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using IGF1R-Beta mouse mAb with DAB staining.
IGF1R-Beta Antibody - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
IGF1R(insulin-like growth factor 1 receptor), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines. Upon binding to its ligands, IGF-I and IGF-II, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for kinase activation.Insulin receptors (IRs) share significant similarity with IGF1 receptors in both structure and function,including an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151).Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation.
REFERENCES
1. Zhu Z. Jiang W. Thompson HJ. Carcinogenesis. 2003, Jul, 24(7):1225-31. Epub 2003 May 9.
2. Ling Y. Maile LA. Clemmons DR. Mol Endocrinol. 2003, Sep,17(9):1824-33. Epub 2003 Jun 5.
