|Application ||WB, E|
|Description||Chemiluminescent detection systems have emerged as the best all-around method for detection of Western blots. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Because results are generated on film, it is possible to record and store data permanently, and blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP (Horseradish Peroxidase) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.|
|Immunogen||Purified recombinant fragment of HRP expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Dilution||WB~~1/500 - 1/2000|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HRP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Villegas J. Cell Tissue Res. 2004,Mar, 315(3):349-59. Epub 2004 Jan 15. 2. Metelitza DI. Karasyova EI. Grintsevich EE. et al. J Inorg Biochem. 2004,Jan, 98(1):1-9.
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