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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CRYAB Antibody   

CRYAB Antibody

Purified Mouse Monoclonal Antibody

  • WB - CRYAB Antibody AO1152a
    Figure 1: Western blot analysis using CRYAB mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CRYAB cDNA (2).
  • IHC - CRYAB Antibody AO1152a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human brain hippocampus (A), lung cancer (B), brain tumor (C), breast cance (D), showing cytoplasmic localization with DAB staining using CRYAB mouse mAb.
  • IHC - CRYAB Antibody AO1152a
    Figure 3: Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissues using CRYAB mouse mAb.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P02511
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1D11C6E6
Isotype IgG2a
Calculated MW 20159 Da
Description Crystallin, alpha B. Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
Immunogen Purified recombinant fragment of CRYAB (aa1-175) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 1410
Other Names Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, CRYAB, CRYA2
Dilution WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCRYAB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms CRYA2
Function May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.
Cellular Location Cytoplasm. Nucleus. Note=Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles
Tissue Location Lens as well as other tissues.
Research Areas
Citations (0)

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1. Cell. 2007 Aug 10;130(3):427-39. 2. Biochemistry. 2006 Nov 21;45(46):13847-54. 3. J Mol Biol. 2007 Sep 14;372(2):470-84.

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$ 385.00
Cat# AO1152a
(40 western blots)
Availability: 7-10 days
Bulk Size
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