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Calnexin Antibody

Purified Mouse Monoclonal Antibody

  • WB - Calnexin Antibody AO1158a
    Figure 1: Western blot analysis using Calnexin mouse mAb against A431 (1), Hela (2), MCF-7 (3) and A549 (4) cell lysate.
  • IF - Calnexin Antibody AO1158a
    Figure 2: Confocal immunofluorescence analysis of Hela cells using Calnexin mouse mAb (green).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P27824
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3H4A7
Isotype IgG2b
Calculated MW 90kDa
Description This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described.
Immunogen Synthetic peptide corresponding to aa (CEAAEERPWLWVVYILTVAL) of human Calnexin, conjugated to KLH.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 821
Other Names Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX
Dilution WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCalnexin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor- mediated endocytosis at the synapse.
Cellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. The palmitoylated form preferentially localizes to the perinuclear rough ER
Citations (0)

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1. Science. 2003 Feb 28;299(5611):1394-7. 2. Exp Cell Res. 2004 Mar 10;294(1):244-53. 3. Science. 2004 Apr 23;304(5670):600-2.

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$ 385.00
Cat# AO1158a
(40 western blots)
Availability: 7-10 days
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