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GABPA Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - GABPA Antibody AO1188a
    Figure 1: Western blot analysis using GABPA mouse mAb against Hela (1), A549 (2), MCF-7 (3), NIH/3T3 (4) and SMMC-7721 (5) cell lysate.
    detail
  • WB - GABPA Antibody AO1188a
    Figure 2: Western blot analysis using GABPA mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GABPA cDNA (2).
    detail
  • IF - GABPA Antibody AO1188a
    Figure 3: Confocal immunofluorescence analysis of Hela cells using GABPA mouse mAb (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC, E
Primary Accession Q06546
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 8C1B10
Isotype IgG1
Calculated MW 51kDa
Description GABPA: GA binding protein transcription factor, alpha subunit 60kDa. It is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, it may play a role in the Down Syndrome phenotype.
Immunogen Purified recombinant fragment of human GABPA (aa120-190) expressed in E. Coli.

Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 2551
Other Names GA-binding protein alpha chain, GABP subunit alpha, Nuclear respiratory factor 2 subunit alpha, Transcription factor E4TF1-60, GABPA, E4TF1A
Dilution WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGABPA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GABPA
Synonyms E4TF1A
Function Transcription factor capable of interacting with purine rich repeats (GA repeats). Positively regulates transcription of transcriptional repressor RHIT/ZNF205 (PubMed:22306510).
Cellular Location Nucleus.
Research Areas
Citations (0)
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References

1. Science. 1998 Feb 13;279(5353):1037-41. 2. J Biol Chem. 1999 Dec 10;274(50):35475-82. 3. EMBO J. 2000 Feb 15;19(4):683-90.

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$ 325.00
Cat# AO1188a
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