|Application ||WB, E|
|Calculated MW||142101 Da|
|Description||MAP4K4: mitogen-activated protein kinase kinase kinase kinase 4. The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.|
|Immunogen||Purified recombinant fragment of MAP4K4(aa400-500) expressed in E. Coli.|
|Other Names||Mitogen-activated protein kinase kinase kinase kinase 4, HPK/GCK-like kinase HGK, MAPK/ERK kinase kinase kinase 4, MEK kinase kinase 4, MEKKK 4, Nck-interacting kinase, MAP4K4, HGK, KIAA0687, NIK|
|Target/Specificity||Purified recombinant fragment of MAP4K4(aa400-500) expressed in E. Coli.|
|Format||Ascitic fluid containing 0.03% sodium azide.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MAP4K4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||HGK, KIAA0687, NIK|
|Function||Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.|
|Tissue Location||Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
1. Mol Cell Biol. 2000 Mar;20(5):1537-45.
2. Curr Biol. 2002 Apr 16;12(8):622-31.
3. J Biol Chem. 2007 Mar 16;282(11):7783-9.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.