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ALCAM Antibody

Purified Mouse Monoclonal Antibody

  • IHC - ALCAM Antibody AO1190a
    Figure 1: Immunohistochemical analysis of paraffin-embedded human ovary carcinoma (A), kidney carcinoma (B), lung carcinoma (C) and breast carcinoma (D), showing cytoplasmic and membrane localization with DAB staining using ALCAM mouse mAb.
  • WB - ALCAM Antibody AO1190a
    Figure 1: Western blot analysis using FRK mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY FRK cDNA (2).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13740
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 10F1G12
Isotype IgG1
Calculated MW 65102 Da
Description ALCAM(CD166): Activated leukocyte cell adhesion molecule.CD166 is a member of the Ig superfamily and is expressed on activated T-cells, B cells and other cells including thymic epithelial cells, fibroblasts, keratinocytes and neurons. CD6 has been identified as a receptor for CD166.
Immunogen Purified recombinant fragment of ALCAM (aa405-524) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 214
Other Names CD166 antigen, Activated leukocyte cell adhesion molecule, CD166, ALCAM, MEMD
Dilution IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALCAM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MEMD
Function Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines.
Research Areas
Citations (0)

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1. Prostate. 2003 Jan 1;54(1):34-43. 2. J Clin Endocrinol Metab. 2003 Jul;88(7):3437-43. 3. J Cell Sci. 2004 Jun 1;117(Pt 13):2841-52. 4. Med Sci Monit. 2006 Aug;12(8):BR263-73.

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$ 300.00
Cat# AO1190a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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