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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   ALCAM Antibody   

ALCAM Antibody

Purified Mouse Monoclonal Antibody

     
  • IHC - ALCAM Antibody AO1190a
    Figure 1: Immunohistochemical analysis of paraffin-embedded human ovary carcinoma (A), kidney carcinoma (B), lung carcinoma (C) and breast carcinoma (D), showing cytoplasmic and membrane localization with DAB staining using ALCAM mouse mAb.
  • WB - ALCAM Antibody AO1190a
    Figure 1: Western blot analysis using FRK mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY FRK cDNA (2).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC, E
Primary Accession Q13740
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 10F1G12
Isotype IgG1
Calculated MW 65102 Da
Description ALCAM(CD166): Activated leukocyte cell adhesion molecule.CD166 is a member of the Ig superfamily and is expressed on activated T-cells, B cells and other cells including thymic epithelial cells, fibroblasts, keratinocytes and neurons. CD6 has been identified as a receptor for CD166.
Immunogen Purified recombinant fragment of ALCAM (aa405-524) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 214
Other Names CD166 antigen, Activated leukocyte cell adhesion molecule, CD166, ALCAM, MEMD
Dilution IHC~~1/200 - 1/1000
WB~~1:500~~2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALCAM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALCAM
Synonyms MEMD {ECO:0000303|PubMed:9502422}
Function Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed:7760007, PubMed:15496415, PubMed:15048703, PubMed:16352806, PubMed:23169771, PubMed:24945728). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed:15048703, PubMed:16352806, PubMed:24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed:15294938, PubMed:16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed:15496415, PubMed:16352806). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed:23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:15496415, PubMed:23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).
Cellular Location Cell membrane; Single- pass type I membrane protein. Cell projection, axon {ECO:0000250|UniProtKB:Q61490}. Cell projection, dendrite {ECO:0000250|UniProtKB:Q61490}. Note=Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells (PubMed:15294938, PubMed:16352806) Colocalizes with CD6 and the TCR/CD3 complex at the immunological synapse (PubMed:15294938).
Tissue Location Detected on hematopoietic stem cells derived from umbilical cord blood (PubMed:24740813). Detected on lymph vessel endothelial cells, skin and tonsil (PubMed:23169771) Detected on peripheral blood monocytes (PubMed:15048703). Detected on monocyte-derived dendritic cells (at protein level) (PubMed:16352806). Detected at low levels in spleen, placenta, liver (PubMed:9502422). Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells (PubMed:7760007). Isoform 1 and isoform 3 are detected in vein and artery endothelial cells, astrocytes, keratinocytes and artery smooth muscle cells (PubMed:15496415). Expressed by neurons in the brain. Restricted expression in tumor cell lines. Detected in highly metastasizing melanoma cell lines (PubMed:9502422)
Research Areas
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References

1. Prostate. 2003 Jan 1;54(1):34-43. 2. J Clin Endocrinol Metab. 2003 Jul;88(7):3437-43. 3. J Cell Sci. 2004 Jun 1;117(Pt 13):2841-52. 4. Med Sci Monit. 2006 Aug;12(8):BR263-73.

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$ 300.00
Cat# AO1190a
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